Degradation of Phenylalanine and Tyrosine by Sporobolomyces
نویسندگان
چکیده
Ammonia-lyase activity for L-phenylalanine, m-hydroxyphenylalanine and L-tyrosine was demonstrated in cell-free extracts ofSporobolomyces roseus. Cultures of this organism converted DL-[ring-14C]phenylalanine and L-[U-14C]tyrosine into the corresponding cinnamic acid. Tracer studies showed that these compounds were further metabolized to [14C]protocatechuic acid. Benzoic acid andp-hydroxybenzoic acid were intermediates in this pathway. Washed cells of the organism readily utilized cinnamic acid, p-coumaric acid, caffeic acid, benzoic acid and p-hydroxybenzoic acid. Protocatechuic acid was the terminal aromatic compound formed during the metabolism of these compounds. The cells ofS. roseus were able to convert m-coumaric acid into m-hydroxybenzoic acid, but the latter compound, which accumulated in the medium, was not further metabolized. 4-Hydroxycoumarin was identified as the product of o-coumaric acid metabolism by this organism.
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